Fig. 2

CrebH deficiency accelerates IBD-associated liver damage. A Representative liver aspect of WT and CrebH^−/− mice treated with 2.5% DSS for 7 days. B, C ALT and AST levels were measured in the plasma of WT (n = 7) and CrebH^−/− (n = 8) mice. ***P < 0.001. D Representative liver histology of WT and CrebH^−/− mice were stained with H&E. The bar represents 200 μm. E The levels of IL-1β, IL-6, and TNFα in the liver from WT (n = 7) and CrebH^−/− (n = 7) mice were analyzed by qRT-PCR. **P < 0.01 or ***P < 0.001. F Liver MPO activity was measured using a commercially available assay kit. **P < 0.01. G Liver images of tunel (left images) and cleaved caspase 3 (right graph) in WT and CrebH^−/− mice. The scale bar represents 200 μm. *P < 0.05, **P < 0.01. Positive cells per field were counted using the ImageJ software. Statistical analysis was performed using a two-tailed Student’s t-test. Error bars represent the mean ± SEM