Fig. 3

The effects of DOR-mediated autophagy on neuronal survival in the rat hippocampus on day 3 postischemia. Western blot analysis of LC3 II/I (a) and P62 (b) levels in the hippocampus (N = 3 per group). c Immunofluorescence images of NeuN-positive neurons in the CA1 region. Obvious neuronal loss was observed in the I/R group, DADLE + naltrindole group, and DADLE + 3-MA group compared with the sham group, while there was an obvious amelioration of neuronal survival in the DADLE group compared to the I/R group. Scale bar = 100 μm. d Quantitative analysis of neuron counts (N = 3 per group). The values are shown as the mean ± SEM. Significant differences: the I/R group vs. the DADLE group (**p < 0.01) and the DADLE group vs. the DADLE + naltrindole group (***p < 0.001) in a; the I/R group and vs. the DADLE group (**p < 0.01) and the DADLE group vs. the DADLE + naltrindole group (***p < 0.001) in b; the I/R group vs. the DADLE group (**p < 0.01) and the DADLE group vs. the DADLE + naltrindole group (**p < 0.01) and the DADLE + 3-MA group (***p < 0.001) in d. DAD DADLE, Nal naltrindole