Fig. 4

CCDC47 regulates calcium flux and its expression may be regulated by intracellular calcium levels in rat H9C2 cardiomyocytes. a CCDC47 overexpressed in rat H9C2 myocytes in the presence of 2 mM calcium show increased cytosolic calcium release induced by 1 µM ionomycin as represented over time. b Maximum calcium release (left graph) and calcium reuptake (right graph) was also higher in cells that overexpressed CCDC47. Bar graphs indicate mean + standard error of n = 3 independent biological replicates. **p < 0.01 and ****p < 0.0001 compared to control (empty vector). c, d Time- and concentration-dependent effect of ionomycin on CCDC47 protein expression. c Representative Western blot and d densitometric analysis. Immunoreactive band intensity for all Western blot data was normalized to β-actin and plotted relative to control. Bar graphs indicate mean + standard error of n = 3 independent biological replicates