Fig. 2

Differentials of the two proteins identified as being localized in the endoplasmic reticulum, regulation of calcium ion homeostasis and calcium ion binding. Primary cardiomyocytes were plated overnight then exposed to high glucose (20 mM) and free fatty acids (oleic acid 3.33 μM, linoleic acid 3.33 μM, and l-carnitine 1 mM or kept under normoglycemic conditions for 6 h prior to harvesting for quantitative proteomic analysis by stable isotope labeling using 8-plex iTRAQ coupled to 2D-LC MALDI MS/MS. All experimental samples were then normalized to a pooled universal reference sample labeled with 113 reagent (as described in “Methods”). a CCDC47 and b Annexin 7. *p < 0.05 compared to cardiomyocytes under normal glucose (control). Bar graphs indicate mean normalized ratio + standard error of n = 5 independent biological samples