Fig. 2

Primary cilia and Arl6 are dynamically regulated during myogenic differentiation of C2C12 cells. a Q-PCR quantification of Arl6 in mouse tissues. The results represent the mean ± SEM of three independent experiments. Quantitation of percentage (b) and ciliary length (c) of ciliated C2C12 cells during myogenic differentiation. Cells were incubated in differentiation medium containing 2% HS for 3 days. The error bars denote SEM. Representative confocal images are shown in Additional file 2: Figure S2. d Q-PCR analysis of mRNA level of Arl6 during differentiation. The data represents the mean ± SEM of three independent experiments. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. 0 h