Fig. 4

The effects of different treatments on endothelial cell (EC) tube formation. A After treatment with the indicated reagents for 6 h, tubular structure in each group was photographed at ×200 magnification. Representative images are shown for the a PBS control; b VEGF; c–e 0.1, 1.0 and 10.0 µM roxarsone, f LY294002 and g LY294002 plus 1.0 µM roxarsone groups. B The number of meshes in five randomly selected images for each group was measured using Image-J with the Angiogenesis Analysis Plugin. The results represent the mean ± SD values from three independent experiments. *p < 0.05, **p < 0.01 vs. the PBS control; ^##p < 0.01 vs. 1.0 µM roxarsone