Anticancer bioactive peptide-3 inhibits human gastric cancer growth by targeting miR-338-5p
© The Author(s) 2016
Received: 18 February 2016
Accepted: 30 June 2016
Published: 22 September 2016
Cancer incidence and mortality have been increasing in China, making cancer the leading cause of death since 2010 and a major public health concern in the country. Cancer stem cells have been studied in relation to the treatment of different malignancies, including gastric cancer. Anticancer bioactive peptide-3 (ACBP-3) can induce the apoptosis of gastric cancer stem cells (GCSCs) and reduce their tumorigenicity. In the present study, for the first time, we used a miRNA microarray and bioinformatics analysis to identify differentially expressed miRNAs in ACBP-3-treated GCSCs and GCSC-derived tumors in a xenograft model and functionally verified the identified miRNAs. miR-338-5p was selected based on its significant upregulation by ACBP-3 both in cultured GCSCs and in tumor tissues.
miR-338-5p was downregulated in GCSCs compared with normal gastric epithelial cells, and the ectopic restoration of miR-338-5p expression in GCSCs inhibited cell proliferation and induced apoptosis, which correlated with the upregulation of the pro-apoptotic Bcl-2 proteins BAK and BIM. We also found that ACBP-3-treated GCSCs could respond to lower effective doses of cisplatin (DDP) or 5-fluorouracil (5-FU), possibly because ACBP-3 induced the expression of miR-338-5p and the BAK and BIM proteins and promoted GCSC apoptosis.
Our data indicate that miR-338-5p is part of an important pathway for the inhibition of human gastric cancer stem cell proliferation by ACBP-3 combined with chemotherapeutics. ACBP-3 could suppress GCSC proliferation and lower the required effective dose of cisplatin or 5-fluorouracil. Therefore, this study provides not only further evidence for the remarkable anti-tumor effect of ACBP-3 but also a possible new approach for the development of GCSC-targeting therapies.
KeywordsmiRNA Microarray miR-338-5p Cisplatin 5-fluorouracil
Gastric cancer (GC) is one of the major threats to human health worldwide. More than 60 % of newly diagnosed cases have been detected in Eastern Asia . Cancer incidence and mortality have been increasing in China, making cancer the leading cause of death since 2010 and a major public health concern in the country. It is predicted that there will be approximately 4,292,000 newly diagnosed invasive cancer cases in 2015 in China, corresponding to almost 12,000 new cancer diagnoses on average each day (The total number of cases projected for 2015 are based on the average incidence rates for the most recent 3 years (2009–2011) using data from 72 population-based cancer registries) . It has been suggested that cancer stem cells (CSCs), found in many types of tumors, and may be responsible for cancer relapse and metastasis. Although the CSC population in tumors is very small, CSCs are thought to underlie the malignant phenotype of tumors, including cancer initiation, angiogenesis, and invasiveness, metastasis, and drug resistance . Although new chemotherapeutic, radio therapeutic and surgical methods have been developed to treat GC, the 5-year survival rate has not improved. One of the main reasons is the existence of gastric cancer stem cells (GCSCs), which can resist chemo- and radiotherapy, escape from apoptosis, and proliferate infinitely , whereas differentiated GC cells are eliminated. Thus, therapeutic approaches targeting GCSCs in GC have been a focus of recent research.
Natural antitumor agents have attracted considerable attention because of their low toxicity and powerful antitumor activity. Anticancer bioactive peptide-3 (ACBP-3), a polypeptide isolated from goat liver in our laboratory, has been shown to inhibit proliferation, induce apoptosis, and reduce the tumorigenicity of human GCSCs both in vitro and in vivo. In addition, ACBP-3 could lower the required effective dose of DDP and improve the condition of mice bearing GCSC-originating tumors . However, the mechanisms underlying the ACBP-3-mediated suppression of GCSCs remains unknown.
MicroRNAs (miRNAs) are small non-coding RNAs of approximately 22 nucleotides that function by targeting mRNAs and regulating gene expression. miRNAs are involved in multiple physiological and pathological processes , including cancer, in which they regulate cell proliferation, differentiation, infiltration, and apoptosis [7, 8]. Thus, miRNA microarrays are widely used as a powerful tool for analyzing the responses of cancer cells to treatments.
Selection of miRNA species differentially expressed in ACBP-3-treated GCSCs
Nine miRNAs selected after rescreening
Fold change (3/1)
miR-338-5p expression in GCSCs and normal gastric epithelial cells
As miR-338-5p demonstrated the highest upregulation by ACBP-3, it was selected for validation by qRT-PCR. We compared the relative miR-338-5p expression in GCSCs and normal human gastric epithelial GES-1 cells and found that the miR-338-5p levels were 1.96 and 1.71 times higher in GES-1 cells than in MKN45- and MKN74G-derived GCSCs, respectively (Fig. 1d).
miR-338-5p-mediated inhibition of GCSC proliferation
Viable cell counting confirmed the above results. At 48 h post-transfection, the cell numbers decreased to 25.33 % ± 2.14 (P < 0.01) and 34.51 % ± 4.02 (P < 0.05), and at 72 h, the cell numbers were 36.05 % ± 2.57 (P < 0.01) and 35.31 % ± 4.11 (P < 0.05) for miR-338-5p-transfected MKN45 and MKN74 GCSCs, respectively, compared with the controls (Fig. 2c, d). However, the miR-338-5p inhibitor did not significantly affect cell viability (P > 0.05).
miR-338-5p induction of GCSC apoptosis
miR-338-5p deregulation of the cell cycle
The effects of miR-338-5p on the cell cycle were assessed in GCSCs 48 h post-transfection by flow cytometry after Annexin V-PI staining. The results indicate that miR-338-5p decreased the proportion of GCSCs in the G0/G1 phase and increased the proportion in the S and G2/M phases (P < 0.01), whereas the miR-338-5p inhibitor did not cause significant cell cycle changes in GCSCs (Fig. 3c–f). Another result was reported in a recent study, in which the transfection of miR-199a-5p inhibitors increased the expression of CTGF and promoted the viability of the cells by increasing the fraction of cells in the G2/M and S phases . Another study reported the Cadmium (Cd)-induced reduction of the number of G0/G1 phase cells and an increase in the number of cells in S phase and G2/M phase, which showed that Cd-induced ROS formation promoted HMGA2 upregulation, causing changes in the cell cycle . Therefore, the over-expression of miR-338-5p decreased the number of GCSCs in G0/G1 phase and increased the number of GCSCs in S and G2/M phase, causing changes in the cell cycle changes and making the cells more likely to be inhibited by chemotherapy drugs. Thus, miR-338-5p, as an anti-cancer gene, can inhibit GCSC proliferation.
Expression of BIM and BAK in GCSCs treated with different drug combinations
Given that miR-338-5p overexpression accelerated GCSC apoptosis and that ACBP-3 (alone or in combination with chemotherapeutic drugs) potently upregulated miR-338-5p levels, we investigated the expression of the pro-apoptotic proteins BIM and BAK in GCSCs treated with ACBP-3, DDP and 5-FU, alone or in combination. The protein expression of BIM and BAK in GCSCs treated with 5-FU, DDP groups, 5-Fu/ACBP-3, DDP/ACBP-3, ACBP-3 or a control treatment for 48 h is shown in Fig. 4c, d. β-actin was used as a reference for the relative level of BIM (Additional file 1: Figure S6 E, G) and BAK (Additional file 1: Figure S6 F, H) in MKN45 cells (Additional file 1: Figure S6 E, F) and MKN74 cells (Additional file 1: Figure S6 G, H). The results showed that in GCSCs, the expression of BIM and BAK positively correlated with that of miR-338-5p. Thus, the BIM and BAK levels were the highest after treatment with ACBP-3, followed by its combination with chemotherapeutic drugs and 5-FU or DDP alone. These results suggest that miR-338-5p could play an important role in the anti-GCSC effects of ACBP-3 by inducing GCSC apoptosis via the upregulation of BIM and BAK expression.
Determination of experimental doses for chemotherapeutic drugs
IC50 values of chemotherapeutic drugs for MKN45-derived GCSCs (μg/mg) and MKN74-derived GCSCs (μg/ml)
ACBP-3 potentiation of DDP and 5-FU cytotoxicity for GCSCs
GCSCs were treated with 5-FU and DDP at a concentration of 0.5 IC50 alone or in combination with 22 μg/ml ACBP-3  and analyzed for the IR. The results showed that in MKN45-derived GCSCs, both drugs combined with ACBP-3 caused significantly stronger inhibition of cell proliferation than when the drugs were used alone (P < 0.01; Fig. 6a, b), whereas in MKN74-derived GCSCs, DDP in combination with ACBP-3 inhibited cell proliferation more effectively than when the drug was used alone (P < 0.01; Fig. 6a, b). However, the combination of 5-FU with ACBP-3 had the same effect on MKN74-derived GCSCs as 5-FU alone, although it was higher than that of ACBP-3 alone (P < 0.01).
Relative miR-338-5p expression in GCSCs treated with different drug combinations
The above results confirmed that ACBP-3 potentiated the anti-GCSC activity of DDP and 5-FU, which is consistent with the findings in vivo . To address the mechanism underlying the observed synergy, we investigated miR-338-5p expression in drug-treated GCSCs. The results indicated that in all treated MKN45- and MKN74-derived GCSCs, miR-338-5p expression was higher compared with the control (P < 0.01 and P < 0.05, respectively). ACBP-3 alone increased the miR-338-5p levels more than 10 times compared with the control (P < 0.01) and significantly elevated the miR-338-5p levels in combination with 5-FU or DDP compared with the drugs alone (P < 0.01; Fig. 6c, d). These results suggest that miR-338-5p upregulation by ACBP-3 may be the mechanism responsible for the ACBP-3-mediated potentiation of the cytotoxic effects of drugs against GCSCs.
CSCs can self-renew for a long time and generate differentiated cancer cell progeny  because of impaired cell cycle checkpoint control and apoptosis regulation as well as decreased DNA sensitivity to chemotherapy and radiotherapy effects due to the upregulation of multidrug resistance proteins . CSCs can divide asymmetrically to generate differentiated cancer cells or symmetrically to maintain a stable population of their own, which accounts for their resistance to cytotoxic drugs [14, 15] and radiation . A sudden decrease in tumor size due to radical treatments may change the microenvironment and activate CSCs , leading to tumor relapse and metastasis . Therefore, effective CSC-targeting therapies would significantly improve cancer prognosis.
Using MKN45 and MKN74 cells, which have the highest GCSC population among human GC cell lines , we have found that ACBP-3 exerted GCSC-suppressive effects in vitro and in vivo. Here, we observed that miR-338-5p was highly upregulated by ACBP-3 in cultured GCSCs and GCSC-derived tumors. miR-338 is considered a central nervous system-specific miRNA encoded in the intron of the AATK (Apoptosis-associated Tyrosine Kinase) gene , which is upregulated during the apoptosis of myeloid precursor cells , and cerebellar granule neurons cultured in low-potassium conditions . In miRNA biogenesis, one strand is loaded in the RNA-induced silencing complex, whereas the other is destroyed ; but for miR-338, both miR-338-3p and miR-338-5p work as transcriptional repressors [24–26]. Previous studies have indicated that miR-338 and miR-338-3p are downregulated in gastric cancer [27, 28], esophageal squamous carcinoma [29, 30], hepatocellular carcinoma [31, 32], and neuroblastoma  and upregulated in oral cancer , pancreatic cancer , and osteosarcoma , suggesting cancer type-specific effects. However, the activity of miR-338-5p has been rarely reported, and the functional relationship between miR-338-3p and miR-338-5p remains unclear.
The present study revealed that miR-338-5p was downregulated in GCSCs compared with normal gastric epithelial cells, suggesting that miR-338-5p may function as an inhibitor of GC development. Given that ACBP-3 induced miR-338-5p in GCSCs and GCSC-initiated tumors, the inhibitory effect of ACBP-3 on GCSCs could be due to the upregulation of miR-338-5p expression. ACBP-3 also inhibited proliferation and promoted apoptosis in GCSCs, which directly correlated with miR-338-5p upregulation, as demonstrated in miR-338-5p-transfected GCSCs . However, a miR-338-5p inhibitor did not show any effects, probably because the starting level of miR-338-5p expression was already downregulated to the baseline and could not be functionally inhibited any further.
Interestingly, in contrast to cell proliferation and viability inhibition, a miR-338-5p mimic induced the transition of GCSCs to the S or G2/M phase of the cell cycle, i.e., accelerated GCSC division. GCSCs have considerable resistance to chemotherapeutic agents due to their relative quiescence and secluded location. It is possible that miR-338-5p facilitates GCSC division and conversion into differentiated GC cells, which are more susceptible to chemotherapy, thus changing quiescent GCSCs to a more vulnerable state. miR-338-5p may first induce GCSCs to divide and differentiate and then promote their apoptosis via unrelated mechanisms. In previous studies, GCSCs were found to be enriched in CD44+ MKN45 and MKN74 cells [5, 19], whereas ACBP-3 could decrease the ratio of CD44+/CD44− cells, which provides support for our hypothesized mechanism of miR-338-5p activity. Further experiments are required to test these speculations.
BAK and BIM are pro-apoptotic members of the BCL-2 family, which controls the mitochondrial apoptotic pathway . The results indicated that BAK and BIM levels correlated with miR-338-5p expression in GCSCs, suggesting that these proteins may be induced by ACBP-3 via miR-338-5p upregulation and then trigger the apoptosis of GCSCs. This mechanism can provide an explanation for the anticancer effects of ACBP-3 related to GCSC apoptosis .
DDP and 5-FU are representative platinum-based and fluorouracil-based chemotherapeutic drugs widely used as first-line anti-cancer agents in the clinic. However, they exert severe side effects, including myeloid suppression, hepatotoxicity, ototoxicity, gastrointestinal toxicity, nephrotoxicity, and peripheral neuropathy , which are exacerbated if different drugs are combined. Our previous results indicated that ACBP-3 could lower the effective dose of DDP  and improve the quality of life of tumor-bearing mice [5, 39]. In this study, we observed that the combination of ACBP-3 with DDP and 5-FU at 0.5 IC50 resulted in stronger inhibition of GCSC proliferation compared with the drugs alone; in addition, the potentiating effect correlated with miR-338-5p expression. As our results indicate, the anticancer efficiency of DDP or 5-FU, which is much stronger than that of ACBP-3, does not depend on miR-338-5p expression, suggesting other cancer-suppressive mechanisms, which may not be sufficient to overcome GCSC resistance. Therefore, the combination of these mechanisms and miR-338-5p upregulation exerted by ACBP-3 may be efficient against GCSCs.
ACBP-3 is a natural compound with low in vivo cytotoxicity . Combined with strong anti-cancer chemotherapeutic agents, it may lower their effective dose by increasing miR-338-5p levels in GCSCs and increasing their sensitivity to chemotherapy while reducing the side effects of the drugs. In a previous study, we conducted a preliminary analysis of the composition of ACBP-3 . In the future, we plan to identify each component and perform detailed functional evaluation, as well as provide further mechanistic insights into ACBP-3 activity.
Our results show, for the first time, that miR-338-5p plays an important role in the inhibition of human gastric cancer stem cell proliferation by ACBP-3 combined with chemotherapeutics. ACBP-3 upregulated miR-338-5p expression, increased BAK and BIM levels, promoted apoptosis and inhibited the proliferation of GCSCs. Our results suggest that ACBP-3 could lower the effective dose of DDP or 5-FU, providing a new direction for CSC-targeting therapy.
ACBP-3 was isolated from goat liver as previously described .
Isolation of GCSCs from human GC cells
GC MKN45 cells were purchased from the Cell Resource Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College (Beijing, China). MKN74 and GES-1 cells were a gift from the laboratory of Prof. Yang K (Health Center at the Peking University College of Medicine, Beijing, China). The cells were cultured in RPMI-1640 (Gibco BRL, Gaithersburg, MD, USA) with 1 % penicillin–streptomycin and 10 % fetal bovine serum (FBS; JRH Biosciences, Lenexa, KS, USA) at 37 °C in a humidified 5 % CO2 incubator. Spheroid colony-derived cells, which constitute a very small proportion of MKN45 and MKN74 GC cells, were identified as GCSCs according to a published method . GCSCs from MKN45 cells were treated with 22 μg/ml ACBP-3 or the same volume of PBS (control). The authenticity of these cell lines was confirmed by the Cell Resource Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College. No authentication of these cell lines was performed by the authors.
Total RNA extraction
Total RNA was extracted from the treated GCSCs and tumor tissues using Trizol (Invitrogen, Paisley, UK) according to the manufacturer’s instructions. RNA concentration was determined using a NanoDrop 2000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA), and its integrity was confirmed by denaturing gel electrophoresis.
miRNA expression profiling was performed using Affymetrix GeneChip miRNA 3.0 arrays (Affymetrix, Santa Clara, CA, USA) containing 19,931 human mature miRNAs in miRBase 17 (http://www.mirbase.org). miRNA expression data are available from the NCBI Gene expression omnibus (GEO). Data processing including gene ontology (GO) analysis , pathway analysis [42, 43], miRNA-gene-network analysis [44–46] and miRNA-GO-network analysis was performed using the Affymetrix Expression Console software (version 1.2).
Quantitative real-time PCR
Selected miRNAs were validated by qRT-PCR. Specific miRNA primers were provided by TIANGEN Biotech (Beijing) Co., Ltd. (Beijing, China), and qRT-PCR was performed using SYBR-Green PCR Master Mix (Takara) and a MX3000P qRT-PCR system (Strata gene) according to the manufacturer’s recommendations. U6 small nuclear RNA was used as a reference gene for normalization. Relative miRNA expression was calculated using the ΔCT method .
Overexpression and inhibition of the target miRNA in GCSCs
GCSCs isolated from MKN45 and MKN74 cells were seeded in 96-well plates at 3 × 105 cells/well in antibiotic-free Opti-MEM supplemented with 10 % FBS and transiently transfected with miR-338-5p mimic or its inhibitor (RIBOBIO, Guangzhou, China) using Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions; vector-transfected cells served as a control. After 24 h of incubation at 37 °C in a humidified 5 % CO2 incubator, the cells were changed into fresh complete medium and cultured for different times.
GCSC proliferation, apoptosis, and cell cycle analysis
After GCSCs were transfected for 24, 48, and 72 h, 1-(4, 5-dimethylthiazol-2-yl)-3, 5-diphenylformazan thiazolyl blue formazan (MTT formazan; Sigma, St. Louis, MO, USA) was added to the wells and incubated at 37 °C for 5 h. Then, 150 μl DMSO was added to each well; the 96-well plates were rotated for 10 min to dissolve the purple crystals, and the absorbance at 490 nm was measured using an ELISA plate reader (Biotek Instruments, Highland Park, VT, USA). Apoptosis and the cell cycle were analyzed in GCSCs transfected for 48 h using the Annexin V Apoptosis Detection Kit (eBioscience, San Diego, CA, USA) and ribonuclease (Worthington Biochemical Corp, Lakewood, NJ, USA) according to the manufacturer’s instructions using a flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA).
Western blot detection of apoptotic proteins
GCSCs transfected with miRNAs or treated with drugs were harvested, lysed with lysis buffer (1 % Triton X-100, 1 mM EGTA, 0.5 % NP-40, 10 mM HEPES (pH 7.4), 1 mM EDTA, 0.15 M NaCl) supplemented with protease inhibitor cocktail (Cell Signaling Technology, Beverly, MA, USA), and sonicated on ice for 10 s. After incubation on ice for 30 min, the lysate was centrifuged at 13,000 rpm for 15 min at 4 °C, the supernatant was collected, and the protein concentration was determined using the Bicinchoninic Acid (BCA) Protein Assay Kit (Pierce, Rockford, IL, USA). Then, 50 g of each lysate was boiled in 5× loading buffer (50 % glycerol, 1 M Tris–HCl, pH 6.8, 10 % β-mercaptoethanol, 10 % SDS, 1 % bromophenol blue), cooled on ice, and separated by SDS-PAGE in 12 % gels. The resolved proteins were analyzed by western blotting using anti-BIM and anti-BAK antibodies (Abcam, Cambridge, MA, USA) and their corresponding secondary antibodies.
Dose determination for chemotherapeutic drugs
GCSCs were seeded in complete medium in 96-well plates in sextuplicate for 24 h and then treated with 2.5–60 μg/ml 5-FU (Jinyao Pharmaceutical Co., Ltd, Tianjin, China) or 1–21 μg/ml DDP (Haosen Pharmaceutical Co., Ltd., Jiangsu, China). Cultures treated with normal saline served as a control. The cell viability was determined after 24, 48, 72 h by the MTT method, and the absorbance at 490 nm was measured using an ELISA plate reader. The inhibition rate (IR) was calculated as (1−A/B) × 100 %, where A and B are the absorbance values of drug-treated and control cells, respectively, and was used to determine the IC50 for each drug.
We used the random-variance model (RVM) t test to compare two experimental groups and the RVM F test to compare more than two groups. The RVM model is used to analyze microarray data and screen for differentially expressed genes because it effectively raises the degrees of freedom for small samples. After the significance and false discovery rate (FDR) analyses, we selected differentially expressed genes according to a P value considered significant at less than 0.05 [48–50].
anticancer bioactive peptide-3
gastric cancer stem cells
cancer stem cells
false discovery rate
Conception and design: XLS, ZWX and LY. Subject recruitment: XL, ZWX, and XLS. Data acquisition: ZWX, LY, XL and XLS. Analysis and interpretation of data: ZWX, LY, XL and XLS. Drafting of the manuscript: ZWX, LY, XL and XLS. Study supervision: XLS. All authors read and approved the final manuscript.
We are grateful to all participants in this study. This work was supported by a grant from the National Natural Science Foundation of China (#81260058 and #81450047).
The authors declare that they have no competing interests.
Availability of data and material
Please see the Additional data.
All sources of funding for the research from the National Natural Science Foundation of China (#81260058 and #81450047).
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Jemal A, Bray F, Center MM, Ferlay J, Ward E, Forman D. Global cancer statistics. CA Cancer J Clin. 2011;61(2):69–90.View ArticlePubMedGoogle Scholar
- Chen WQ, Zheng RS, Baade PD, Zhang SW, Zeng HM, Bray F, et al. CA Cancer J Clin. 2016. doi: 10.3322/caac.21338.
- Signore M, Ricci-Vitiani L, De Maria R. Targeting apoptosis pathways in cancer stem cells. Cancer Lett. 2013;332(2):374–82.View ArticlePubMedGoogle Scholar
- Burnett J, Newman B, Sun D. Targeting cancer stem cells with natural products. Curr Drug Targets. 2012;13(8):1054–64.View ArticlePubMedGoogle Scholar
- Yu L, Yang L, An W, Su X. Anticancer bioactive peptide-3 inhibits human gastric cancer growth by suppressing gastric cancer stem cells. J Cell Biochem. 2014;115(4):697–711.View ArticlePubMedGoogle Scholar
- Bartel DP. MicroRNAs: genomics, biogenesis, mechanism, and function. Cell. 2004;116(2):281–97.View ArticlePubMedGoogle Scholar
- Lee HC, Kim JG, Chae YS, Sohn SK, Kang BW, Moon JH, et al. Prognostic impact of microRNA-related gene polymorphisms on survival of patients with colorectal cancer. J Cancer Res Clin Oncol. 2010;136(7):1073–8.View ArticlePubMedGoogle Scholar
- Mosakhani N, Sarhadi VK, Borze I, Karjalaineu-Lindsberg ML, Sundstrom J, Ristamaki R, et al. MicroRNA profiling differentiates colorectal cancer according to KRAS status. Genes Chromosomes Cancer. 2012;51(1):1–9.View ArticlePubMedGoogle Scholar
- Xie H, Wang J, Jiang L, Geng C, Li Q, Mei D, et al. ROS-dependent HMGA2 upregulation mediates Cd-induced proliferation in MRC-5 cells. Toxicol In Vitro. 2016;S0887–2333(16):30068.Google Scholar
- Sun D, Han S, Liu C, Zhou R, Sun W, Zhang Z, et al. Microrna-199a-5p functions as a tumor suppressor via suppressing connective tissue growth factor (CTGF) in follicular thyroid carcinoma. Med Sci Monit. 2016;22:1210–2117.View ArticlePubMedPubMed CentralGoogle Scholar
- Xu Z, Sharp PP, Yao Y, Segal D, Ang CH, Khaw SL, et al. BET inhibition represses miR17-92 to drive BIM-initiated apoptosis of normal and transformed hematopoietic cells. Leukemia. 2016. doi:10.1038/leu.2016.52.PubMed CentralGoogle Scholar
- García Sáez AJ, Villunger A. MOMP in the absence of BH3-only proteins. Genes Dev. 2016;30(8):878–80.View ArticlePubMedGoogle Scholar
- Giannakis M, Stappenbeck TS, Mills JC, Leip DG, Lovett M, Clifton SW, et al. Molecular properties of adult mouse gastric and intestinal epithelial progenitors in their niches. J Biol Chem. 2006;281(16):11292–300.View ArticlePubMedGoogle Scholar
- Bertolini G, Roz L, Perego P, Tortoreto M, Fontanella E, Gatti L, et al. Highly tumorigenic lung cancer CD133 + cells display stem-like features and are spared by cisplatin treatment. Proc Natl Acad Sci USA. 2009;106(38):16281–6.View ArticlePubMedPubMed CentralGoogle Scholar
- Dylla SJ, Beviglia L, Park IK, Chartier C, Raval J, Nqan L, et al. Colorectal cancer stem cells are enriched in xenogeneic tumors following chemotherapy. PLoS ONE. 2008;3(6):e2428.View ArticlePubMedPubMed CentralGoogle Scholar
- Diehn M, Cho RW, Lobo NA, Kalisky T, Dorie MJ, Kulp AN, et al. Association of reactive oxygen species levels and radioresistance in cancer stem cells. Nature. 2009;458(7239):780–3.View ArticlePubMedPubMed CentralGoogle Scholar
- Chefetz I, Alvero AB, Holmberg JC, Lebowitz N, Craveiro V, Yang-Hartwich Y, et al. TLR2 enhances ovarian cancer stem cell self-renewal and promotes tumor repair and recurrence. Cell Cycle. 2013;12(3):511–21.View ArticlePubMedPubMed CentralGoogle Scholar
- Salcido CD, Larochelle A, Taylor BJ, Dunbar CE, Varticovski L. Molecular characterisation of side population cells with cancer stem cell-like characteristics in small-cell lung cancer. Br J Cancer. 2010;102(11):1636–44.View ArticlePubMedPubMed CentralGoogle Scholar
- Takaishi S, Okumura T, Tu S, Wang SS, Shibata W, Viqneshwaran R, et al. Identification of gastric cancer stem cells using the cell surface marker CD44. Stem Cells. 2009;27(5):1006–20.View ArticlePubMedPubMed CentralGoogle Scholar
- Barik S. An intronic microRNA silences genes that are functionally antagonistic to its host gene. Nucleic Acids Res. 2008;36(16):5232–41.View ArticlePubMedPubMed CentralGoogle Scholar
- Gaozza E, Baker SJ, Vora RK, Reddy EP. AATYK: a novel tyrosine kinase induced during growth arrest and apoptosis of myeloid cells. Oncogene. 1997;15(25):3127–35.View ArticlePubMedGoogle Scholar
- Tomomura M, Fernandez-Gonzales A, Yano R, Yuzaki M. Characterization of the apoptosis-associated tyrosine kinase (AATYK) expressed in the CNS. Oncogene. 2001;20(9):1022–32.View ArticlePubMedGoogle Scholar
- Olde LNF, Kos A, Martens GJ, Van Bokhoven H, Nadif KN, et al. MicroRNA networks direct neuronal development and plasticity. Cell Mol Life Sci. 2012;69(1):89–102.View ArticleGoogle Scholar
- Wu H, Ye C, Ramirez D, Manjunath N. Alternative processing of primary microRNA transcripts by Drosha generates 5′ end variation of mature microRNA. PLoS ONE. 2009;4(10):e7566.View ArticlePubMedPubMed CentralGoogle Scholar
- Zhao X, He X, Han X, Yu Y, Ye F, Chen Y, et al. MicroRNA-mediated control of oligodendrocyte differentiation. Neuron. 2010;65(5):612–26.View ArticlePubMedPubMed CentralGoogle Scholar
- Aschrafi A, Natera-Naranjo O, Gioio AE, Kaplan BB. Regulation of axonal trafficking of cytochrome c oxidase IV mRNA. Mol Cell Neurosci. 2010;43(4):422–30.View ArticlePubMedPubMed CentralGoogle Scholar
- Li P, Chen X, Su L, Li C, Zhi Q, Yu B, et al. Epigenetic silencing of miR-338-3p contributes to tumorigenicity in gastric cancer by targeting SSX2IP. PLoS ONE. 2013;8(6):e66782.View ArticlePubMedPubMed CentralGoogle Scholar
- Guo B, Liu L, Yao J, Ma R, Chang D, Li Z, et al. miR-338-3p suppresses gastric cancer progression through a PTEN-AKT axis by targeting P-REX2a. Mol Cancer Res. 2014;12(3):313–21.View ArticlePubMedGoogle Scholar
- Yang M, Liu R, Sheng J, Liao J, Wang Y, Pan E, et al. Differential expression profiles of microRNAs as potential biomarkers for the early diagnosis of esophageal squamous cell carcinoma. Oncol Rep. 2013;29(1):169–76.PubMedGoogle Scholar
- Wu B, Li C, Zhang P, Yao Q, Wu J, Han J, et al. Dissection of miRNA-miRNA interaction in esophageal squamous cell carcinoma. PLoS ONE. 2013;8(9):e73191.View ArticlePubMedPubMed CentralGoogle Scholar
- Huang XH, Wang Q, Chen JS, Fu XH, Chen XL, Chen LZ, et al. Bead-based microarray analysis of microRNA expression in hepatocellular carcinoma: miR-338 is downregulated. Hepatol Res. 2009;39(8):786–94.View ArticlePubMedGoogle Scholar
- Fu X, Tan D, Hou Z, Hu Z, Liu G, Ouyang Y, et al. The effect of miR-338-3p on HBx deletion-mutant (HBx-d382) mediated liver-cell proliferation through CyclinD1 regulation. PLoS ONE. 2012;7(8):e43204.View ArticlePubMedPubMed CentralGoogle Scholar
- Chen X, Pan M, Han L, Lu H, Hao X, Dong Q. miR-338-3p suppresses neuroblastoma proliferation, invasion and migration through targeting PREX2a. FEBS Lett. 2013;587(22):3729–37.View ArticlePubMedGoogle Scholar
- Maclellan SA, Lawson J, Baik J, Guillaud M, Poh CF, Garnis C. Differential expression of miRNAs in the serum of patients with high-risk oral lesions. Cancer Med. 2012;1(2):268–74.View ArticlePubMedPubMed CentralGoogle Scholar
- Yu J, Li A, Hong SM, Hruban RH, Goggins M. MicroRNA alterations of pancreatic intraepithelial neoplasias. Clin Cancer Res. 2012;18(4):981–92.View ArticlePubMedGoogle Scholar
- Won KY, Kim YW, Kim HS, Lee SK, Jung WW, Park YK. MicroRNA-199b-5p is involved in the Notch signaling pathway in osteosarcoma. Hum Pathol. 2013;44(8):1648–55.View ArticlePubMedGoogle Scholar
- Youle RJ, Strasser A. The BCL-2 protein family: opposing activities that mediate cell death. Nat Rev Mol Cell Biol. 2008;9(1):47–59.View ArticlePubMedGoogle Scholar
- Chu KM, Ngan MP, Wai MK, Yeung CK, Andrews PL, Percie du Sert N, et al. Olvanil, a non-pungent vanilloid enhances the gastrointestinal toxicity of cisplatin in the ferret. Toxicol Lett. 2010;192(3):402–7.View ArticlePubMedGoogle Scholar
- Su X, Dong C, Zhang J, Su L, Wang X, Cui H, et al. Combination therapy of anti-cancer bioactive peptide with Cisplatin decreases chemotherapy dosing and toxicity to improve the quality of life in xenograft nude mice bearing human gastric cancer. Cell Biosci. 2014;4(1):7.View ArticlePubMedPubMed CentralGoogle Scholar
- Su L, Xu G, Shen J, Tuo Y, Zhang X, Jia S, et al. Anticancer bioactive peptide suppresses human gastric cancer growth through modulation of apoptosis and the cell cycle. Oncol Rep. 2010;23(1):3–9.PubMedGoogle Scholar
- Schlitt T, Palin K, Rung J, Dietmann S, Lappe M, Ukkonen E, et al. From gene networks to gene function. Genome Res. 2003;13(12):2568–76.View ArticlePubMedPubMed CentralGoogle Scholar
- Yi M, Horton JD, Cohen JC, Hobbs HH, Stephens RM. Whole Pathway Scope: a comprehensive pathway-based analysis tool for high-throughput data. BMC Bioinform. 2006;7:30.View ArticleGoogle Scholar
- Draghici S, Khatri P, Tarca AL, Amin K, Done A, Voichita C, et al. A systems biology approach for pathway level analysis. Genome Res. 2007;17(10):1537–45.View ArticlePubMedPubMed CentralGoogle Scholar
- Joung JG, Hwang KB, Nam JW, Kim SJ, Zhang BT. Discovery of microRNA-mRNA modules via population-based probabilistic learning. Bioinformatics. 2007;23(9):1141–7.View ArticlePubMedGoogle Scholar
- Shalgi R, Lieber D, Oren M, Pilpel Y. Global and local architecture of the mammalian microRNA-transcription factor regulatory network. PLoS Comput Biol. 2007;3(7):e131.View ArticlePubMedPubMed CentralGoogle Scholar
- Enright AJ, John B, Gaul U, Tuschl T, Sander C, Marks DS. MicroRNA targets in Drosophila. Genome Biol. 2003;5(1):R1.View ArticlePubMedPubMed CentralGoogle Scholar
- Schmittgen TD, Livak KJ. Analyzing real-time PCR data by the comparative C (T) method. Nat Protoc. 2008;3(6):1101–8.View ArticlePubMedGoogle Scholar
- Wright GW, Simon RM. A random variance model for detection of differential gene expression in small microarray experiments. Bioinformatics. 2003;19(18):2448–55.View ArticlePubMedGoogle Scholar
- Yang H, Crawford N, Lukes L, Finney R, Lancaster M, Hunter KW. Metastasis predictive signature profiles pre-exist in normal tissues. Clin Exp Metastasis. 2005;22(7):593–603.View ArticlePubMedGoogle Scholar
- Clarke R, Ressom HW, Wang A, Xuan J, Liu MC, Gehan EA, et al. The properties of high-dimensional data spaces: implications for exploring gene and protein expression data. Nat Rev Cancer. 2008;8(1):37–49.View ArticlePubMedPubMed CentralGoogle Scholar