Fig. 3

Low MP Cells are enriched for hematopoietic stem cells. In vitro colony forming assay using low and high MP human MPB cells. Panel a to d shows the number of colonies derived from low and high MP cells after 12 days in methocult medium. Note the significantly higher number of colonies derived from the low MP population. (n = 3, p < 0.001) Different types of colonies derived from low and high MP cells were analyzed. b, c and d are the percentages of CFU-GEMM, BFU-E and CFU-GM respectively. e) FACS-sorted low MP cells and high MP cells were injected into the tibia of sublethally irradiated NOD/SCID mice. The donor repopulation was shown after 2 months. f) Real Time PCR array of HPSC markers comparing low MP cells to high MP cells. g) Real Time PCR array of differentiation markers comparing low MP to high MP cells. Note the enrichment of a number of markers for HPSCs and the downregulation of differentiation markers. *p < 0.05, ***p < 0.001