Figure 4

The dynamic actin pattern in sch9Δ, tor1Δ, and ras2Δ cells relies on different effectors. The actin cytoskeleton in sch9Δ depends on the RIM15 and MSN2/4 transcription factors, not GIS1 (A), while tor1Δ and ras2Δ depend on the RIM15 (B, C). Wild type and mutant strains were grown in aging medium. At indicated time points, cells were removed either for testing of cell viability or for fluorescence microscopy. Cells were fixed for 1 h by adding 37% formaldehyde solution directly to the growth medium to a final concentration of 3.7%. After washing with 1× PBS solution for three times, cells were stained with phalloidin-TRITC in the dark at room temperature for 1 h and images were taken by fluorescent microscope. Scale bar, 5 μm.