Induction of the intrinsic apoptotic pathway via a new antimitotic agent in an esophageal carcinoma cell line

Cell & Bioscience

Table 1 Average percentage of cells in interphase, various stages of mitosis and cells displaying apoptotic morphology

	Interphase	Prophase	Metaphase	Anaphase	Telophase	Apoptosis	Abnormal
Medium	96.1%	0.6%	1.5%	0.4%	0.6%	0.5%	0.4%
DMSO	97.5%	0.2%	1.3%	0.2%	0.6%	0.2%	0.4%
Actinomycin D	60.2%	0.3%	2.5%	0%	0.5%	33.8%	2.3%
ESE-16	27.3%	0.7%	46.0%^*	0.2%	0.4%	21.4%^*	4.2%

Mitotic indices revealed a significant increase, with a P-value of 0.0003*, in the percentage of cells in metaphase in the ESE-16-treated samples (46.0%) when compared to the vehicle control (1.3%). ESE-16-treated cells also showed a significant increase (P-value of 0.0006*) in the percentage of cells undergoing apoptosis (21.4%) when compared to the vehicle control (0.2%).

ISSN: 2045-3701