Figure 5From: Interleukin-6 is required for cell cycle arrest and activation of DNA repair enzymes after partial hepatectomy in miceHepatocyte proliferation in vivo was determined using the bromodeoxyuridine (BrdU) incorporation assay. Mice were injected (IP) with BrdU (50 μg/g body weight) and euthanized 2 hours later, and the livers were harvested for IHC staining of BrdU using a kit (BD Biosciences). The percentage of BrdU-labeled hepatocytes in each slide was counted in five low-power (×100) fields. Data represent mean ± SEM (n = 3). *P < 0.05.Back to article page