Figure 4
Increased antigen-stimulated IL-6 and TNF-α expression from SRC-3-/-BMMCs compared with SRC-3+/+BMMCs. BMMCs were pretreated with 1 μg/ml anti-DNP IgE for 4 h and then were stimulated with different concentration of DNP-HSA overnight at 37°C in 5% CO2. (A and B) The amounts of IL-6 and TNF-α in the medium were measured using ELISA assay kits. C and D, The mRNA levels of IL-6 and TNF-α were measured by real-time PCR. Values are shown as the mean + SD from three independent experiments. *p < 0.05.