Figure 3
From: Cell density during differentiation can alter the phenotype of bone marrow-derived macrophages
Expression of macrophage polarization genes in BMDMs. (a) Expression of genes related to the M1 polarization state upon activation with 100 ng/mL LPS for 18 hours and (b) M2 polarization state after treatment with 10 ng/mL of IL-4 (Peprotech) for 18 hours. Total RNA was isolated by GE RNAspin mini kit and reverse transcribed with Super Script II (Invitrogen) and run on ABI 7500 qPCR machine using ABI SYBERGreen Mastermix. Statistical analysis was done by student t test with Welch’s correction where appropriate. Result is a representative of three independent experiments with three biological samples per datapoint. *** P < 0.0005; ** P < 0.005; * P < 0.05.