Figure 4

Autophagy inhibition increased ischemia-induced cell necrosis but not apoptosis in the rat livers. (A) Four groups of rats were treated as indicated. Then their liver sections were detected by immunohistofluorescence staining of HMGB1 (upper) and Tunel staining (lower). Arrow indicated the cells with the release of HMGB1 from the nuclei (upper) and Tunel-positive cells (lower). (B and C) The number of cells without nuclear HMGB1 (B) and Tunel-positive cells (C) per high power field (HP, magnification × 400) were shown in the graph. Data were shown as mean ± SEM (n = 5; *: p < 0.05; **: p < 0.01; ns: no significant difference).