From: Generation of gene disruptions by transcription activator-like effector nucleases (TALENs) in Xenopus tropicalis embryos
Components
pCS2-TALEN Mix (μL)
Buffer 3 (NEB)
1
pFUS_A-RVDs vectors (150 ng/μL)
0.8
pFUS_B-RVDs vectors (150 ng/μL)
Last Repeats vectors (150 ng/μL)
0.6
BsmbI (NEB)
pCS2-TALEN-ELD/KKR vectors (150 ng/μL)
dH2O
5
Total
10