Figure 5

Analyses of the trans expression of miRNAs and antagomirs on the infectivity of the indicated NL4-3 viruses. 293T cells were seeded in a 6-well plate and transfected with 2 μg of proviral plasmid and 100 pM of the indicated RNA. Supernatants were harvested at 24 hours post transfection, filtered and used to determine infectious units as normalized by RT activity. (A) Evaluation of NL4-3 miR326 virus. NL4-3 let7 scr was co-transfected with either control RNA (column 1) or miR326 (column 2). NL4-3 miR326 was co-transfected with either control RNA (column 3) or an anti-miR326 antagomir (column 4). Results are shown relative to the control NL4-3 let7 scr virus. (B) Evaluation of NL4-3 miR211 virus. NL4-3 let7 scr was co-transfected with either control RNA (column 1) or miR211 (column 2). NL4-3 miR211 was co-transfected with either control RNA (column 3) or an anti-miR211 antagomir (column 4). Results are shown relative to the control NL4-3 let7 scr virus. All data are the averages of three replicates. ** indicates a p-value < 0.01.