Figure 6
Phagocytic Activity and LAMP Protein. (A) Comparisons of LAMP-1 and LAMP-2 protein levels at different concentrations of chloroquine. Protein levels measured in ARPE-19 by Western blot with beta-actin as loading control. Western shows marked qualitative increase in LAMP 1-2 band size between control and 10 Ī¼g/ml chloroquine treatment, while loaded control is constant. (B) There is a significant increase in LAMP-1 and 2 (p < 0.05) at 10 Ī¼g/ml with overall upregulation trend response to chloroquine treatment. Immunoblots (n = 6) were scanned, and densitometry was performed on bands with ImageJ (NIH, Bethesda MD). Relative band density measurement was repeated several times to ensure maximum and minimum values.